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EU Assassins Creed 2 (The Ezio Collection) [CUSA04893]

As someone else noted, save 33 in the zip for Vitruvian Man is US region.

Since it doesn't state, so far as I can tell, where the last glyph is... in Venice, "Behind the arch of the roof of Scuola Grande di San Marco's white building."

Interestingly, this save is also at 99/100 feathers, allowing for In Memory of Petruccio and Show your Colors. The last feather is near the glyph. Head east to the blacksmith from the glyph and the last feather is on a wooden beam before you get there.
 
for exact last glyph and feather location in venice

QznKilE.png